The information below serves as a general guide only. Please refer to your quote for project specific sample submission information. If you cannot provide the requested amount please contact us for options.
General Sample Submission Criteria
- Samples should be intact i.e. not degraded.
- Samples should be free of contaminants: 260:280 1.8-2.2 and 260:230 ratio >1.7.
- RNA samples must be free of genomic DNA contamination.
- DNA samples must be free of RNA contamination.
- DNA concentration should be measured using the Qubit or Picogreen assay as quantification by spectrophotometric methods is inaccurate.
Application Specific Sample Submission Criteria
Genome Sequencing - Short Read
Service | Amount Requested | Concentration | Minimum Volume |
---|---|---|---|
PCR-free sample prep (Illumina DNA PCR-free Prep) |
1 ug DNA |
30-60 ng/ul |
30 ul |
PCR-plus sample prep |
500ng DNA |
20-25 ng/ul |
25 ul |
RNA Sequencing - Short Read
Service | Amount Requested | Concentration | Minimum Volume |
---|---|---|---|
mRNA seq |
1.2 ug total RNA |
30-40 ng/ul |
40 ul |
Total RNA seq - human, mouse, rat, bacteria |
400 ng total RNA |
20-50 ng/ul |
20 ul |
Total RNA seq - plants |
3 ug total RNA | 120-180 ng/ul | 25 ul |
Total RNA seq - low input |
10-20 ng total RNA |
1-4 ng/ul |
12 ul |
Total RNA seq - rRNA depleted submission |
120 ng rRNA depleted RNA |
10-20 ng/ul |
12 ul |
small RNA* |
600 ng total RNA |
40-100 ng/ul |
15 ul |
* contact us for input requirements for serum and plasma.
Client Prepared Libraries
- Refer to your quote for the amount for this service.
- Library fragments up to 1kb.
- Custom primers must be supplied at the time of sample submission.
- One pool per run (unless prior arrangements have been made).
Exome and Panel Sequencing
We provide various capture options requiring different input amounts. Contact us for further information.
Microbiome Sequencing
- 15-20ul of DNA at a concentration of 5-10ng/ul.
- Quantification should be performed by a fluorescence assay (Qubit or Picogreen).
- DNA of high purity (260/280, 260/230 >~1.8).
- Samples must be amplifiable by PCR (please check this prior to submission).
ChIP Sequencing
- 5ng of the enriched material in a total volume of 12-16ul.
- Quantification should be performed by a fluorescence assay (Qubit or Picogreen).
- The fragment size range of the enriched material should be 200-600bp.
Oxford Nanopore Long Read Sequencing
- Volumes and concentration depends on the application.Contact us for further information or refer to your quote.
- Our Long & Linked Read Sample Submission Guide provides general information on DNA/RNA quality.
- Samples should be of high purity (260/280 and 260/230 ratios of 2.0-2.2).
- Oxford Nanopore guideline for plasmid extraction.
Genotyping by Microarray
- 50ul of intact genomic DNA at a concentration of 15 - 50 ng/ul.
- Quantification must be performed by a fluorescence assay (Qubit or Picogreen).
- Samples should be resuspended in nuclease free water or low EDTA TE buffer.
- The 260:280 OD ratio should be 1.8 - 2.2 and the 260:230 OD ratio >1.8.
- DNA should be intact as assessed by agarose gel or similar method.
- Note: We can accept dried samples if plate sealing is a concern.
Nanostring Services
Contact us for further information or refer to your quote.