The information below serves as a general guide only. Please refer to your quote for project specific sample submission information. If you cannot provide the requested amount please contact us for options.
General Sample Submission Criteria
- Samples should be intact i.e. not degraded.
- Samples should be free of contaminants: 260:280 1.8-2.2 and 260:230 ratio >1.8.
- RNA samples must be free of genomic DNA contamination.
- DNA samples must be free of RNA contamination.
- DNA concentration should be measured using the Qubit or Picogreen assay as quantification by spectrophotometric methods is inaccurate.
Application Specific Sample Submission Criteria
Genome Sequencing - Short Read
| Service | Amount Requested | Concentration | Minimum Volume |
|---|---|---|---|
| PCR-free sample prep (Illumina DNA PCR-free Prep) | 1 ug DNA | 30-60 ng/ul | 30 ul |
PCR-plus sample prep | 500ng DNA | 20-25 ng/ul | 25 ul |
RNA Sequencing - Short Read
| Service | Amount Requested | Concentration | Minimum Volume |
|---|---|---|---|
mRNA seq | >1.25 ug total RNA | 25-700 ng/ul | 50 ul |
Total RNA seq - human, mouse, rat, bacteria | >500 ng total RNA | 20-50 ng/ul | 20 ul |
Total RNA seq - plants | >2.5 ug total RNA | 100-200 ng/ul | 25 ul |
Total RNA seq - low input | >15 ng total RNA | 1-5 ng/ul | 15 ul |
| small RNA-seq* | >375 ng total RNA | 25-50 ng/ul | 15 ul |
ILMN RNA Enrichment | >500 ng total RNA | 25-50 ng/ul | 20 ul |
Contact us for input requirements for serum and plasma.
Client Prepared Libraries
- Refer to your quote for the amount for this service.
- Library fragments up to 1kb.
- Custom primers must be supplied at the time of sample submission.
- One pool per run (unless prior arrangements have been made).
Exome and Panel Sequencing
We provide various capture options requiring different input amounts. Contact us for further information.
Microbiome Sequencing
- 15-20ul of DNA at a concentration of 5-10ng/ul.
- Quantification should be performed by a fluorescence assay (Qubit or Picogreen).
- DNA of high purity (260/280, 260/230 >~1.8).
- Samples must be amplifiable by PCR (please check this prior to submission).
Epigenetics Services
| Service | Amount Requested | Concentration | Minimum Volume |
|---|---|---|---|
| ChIP-seq (fragment size must be in 200-700bp range) | >4ng | 400pg-4 ng/ul | 12 ul |
Whole Genome Enzymatic Methyl-seq | >500ng ng | 20-25 ng/ul | 20 ul |
*For Reduced Representation Bisulfite Sequencing (RRBS) and targeted Methyl-seq
Whole Plasmid Sequencing using Oxford Nanopore
- >300ng of purified plasmid DNA at a concentration of 15-50ng/ul (measured with Qubit) in nuclease free water or elution buffer.
- Minimum volume 20ul/sample.
- Plasmid size: 2-25kb. Please contact us if the plasmid is outside this size range as this may affect quantity of DNA required.
- 260/280: 1.8 - 2.0
- DNA is double-stranded and not fragmented (degraded).
- The sample does not contain a mixture of plasmids.
- The DNA is clean and does not contain contaminants that may affect prep or sequencing results.
 
For more information, see the Oxford Nanopore guideline for plasmid extraction.
Please note, submitted samples are only checked with qubit. By submitting, the client confirms that the samples conform to the above requirements.
Oxford Nanopore Long Read Sequencing
- Volumes and concentration depends on the application. Contact us for further information or refer to your quote.
- Our Long & Linked Read Sample Submission Guide provides general information on DNA/RNA quality.
- For full-length plasmid sequencing, see above.
Microarray
- Axiom Genotyping: 50ul of intact genomic DNA at a concentration of 15 - 50 ng/ul.
- Infinium Genotyping: 20ul of intact genomic DNA at a concentration of 40 - 60 ng/ul.
- Infinium Methylation array: 45ul of intact genomic DNA at a concentration of 25 - 50 ng/ul.
- Quantification must be performed by a fluorescence assay (Qubit or Picogreen).
- Samples should be resuspended in nuclease free water or low EDTA TE buffer.
- The 260:280 OD ratio should be 1.8 - 2.2 and the 260:230 OD ratio >1.8.
- DNA should be intact as assessed by agarose gel or similar method.
- Note: We can accept dried samples if plate sealing is a concern.
Nanostring Services
Contact us for further information or refer to your quote.