Our Core Prep services are an easy option for researchers who would like to fast track their project or for those who are new to Sanger sequencing.
Core Prep
You submit a mix of purified PCR product and primer, we perform the sequencing reaction and capillary separation.
Turnaround time: 3-5 days.*
Core Prep with Clean Up
You submit unpurified PCR product and primer, we perform the PCR clean up, sequencing reaction and capillary separation. Clean up options include ExoSAp-IT and magnetic bead clean up (plate submissions only).
Turnaround time: 3-5 days.*
Returning Customers
Please log in to our Sanger Sequencing Portal to submit your samples.
New Customers
Please download our user guides for detailed instructions on how to use our Sanger sequencing services:
- Sanger Sequencing Guide – will provide you with a step-by-step guide to sample preparation.
- Sanger Sequencing Online Submission Guide – will walk you through creating an account on our Sanger Sequencing Portal and submitting samples online.
Data Delivery
You will receive an email notification once processing is complete, you will then be able to access your data via your dnaLIMS account. The following files are available to download:
- Raw trace (.ab1 file)
- Raw, unfiltered basecall sequence (.seq file)
- Text file of you sequence
- FASTA Sequence
- System QC score of the sequence
Quality Guarantee
- We include internal controls (pGem) on all sequencing runs to ensure quality data
- All traces are manually inspected before data is released
- Free re-run service if results do not meet our quality standards
* Working days are Monday – Friday (excluding Public Holidays). Samples must be received by 12 noon to be included in the days sequencing run.